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Structure of 1807503-90-7
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Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.Triton X-100 has been used:In immunohistochemistry for staining the Flat-mount retinasAlong with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assayTo permeabilise cells during Immunofluorescent microscopic studiesAs a positive control in LDH assay to determine the cell membrane integrityFor estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedureFor the preparation of outer membrane protein exctractAs a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cellsIn the treatment of tissue sections for Immunofluorescence labeling
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GHS Pictogram :
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GHS No : GHS07
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Signal Word : Warning
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UN#: N/A
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Class : N/A
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Packing Group : N/A
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Hazard Statements : H302-H315-H319-H335
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Precautionary Statements : P260-P264-P270-P271-P280-P301+P310-P302+P352-P304+P340-P305+P351+P338-P312-P330-P332+P313-P337+P313-P362+P364-P403+P233-P405-P501
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Lot numbers can be found on the outside label of a product: