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CS-W001157 4
Total: USD 88.00

Biotin-PFP ester

  • CAS No. 120550-35-8

  • Cat. No. CS-0067557
  • Purity≥95%
  • MDL No.MFCD09952636

*For research and further manufacturing use only, not for direct human use.

Biotin-PFP ester|CS-0067557

Structure of 120550-35-8

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General Information

  • CAS No. 120550-35-8
  • Cat. No. CS-0067557
  • Purity ≥95%
  • MDL No. MFCD09952636
  • Storage 4°C
  • Shipping Room temperature in continental US; may vary elsewhere.
  • Molecular Formula C₁₆H₁₅F₅N₂O₃S
  • Molecular Weight 410.36
  • Synonym(s) None
  • SMILES O=C1N[C@]2(CS[C@H]([C@]2(N1)[H])CCCCC(OC3=C(C(F)=C(C(F)=C3F)F)F)=O)[H]

Description

Biotin-PFP ester enables site-specific protein labeling through rapid, stable amide bond formation with primary amines under physiological conditions. This highly reactive ester derivative features a PFP (pentafluorophenyl) group that ensures efficient conjugation without requiring additional coupling agents. Ideal for bioconjugation workflows in antibody-drug conjugates and affinity purification applications.

Application

Biotin-PFP ester serves as a highly reactive, bench-stable reagent for site-specific protein labeling and biomolecule conjugation. The PFP ester group readily reacts with primary amines under mild aqueous conditions, enabling efficient bioconjugation without requiring additional catalysts. Its high stability in storage and compatibility with diverse reaction media make it ideal for use in immunoassays, affinity purification, and the construction of biotinylated probes for downstream detection via streptavidin systems.

Synthesis

  • Route1

    References

    • Korshun, Vladimir A.; Pestov, Nikolai B.; Nozhevnikova, Elena V.; Prokhorenko, Igor A.; Gontarev, Sergei V.; Berlin, Yuri A. Synthetic Communications, 1996 , vol. 26, # 13 p. 2531 - 2547
  • Route2

    References

    • Chambers, Jennifer M.; Lindqvist, Lisa M.; Webb, Andrew; Huang, David C. S.; Savage, G. Paul; Rizzacasa, Mark A. Organic Letters, 2013 , vol. 15, # 6 p. 1406 - 1409
  • Route3

    References

    • Stewart, Scott G.; Braun, Carlos J.; Polomska, Marta E.; Karimi, Mahdad; Abraham, Lawrence J.; Stubbs, Keith A. Organic and Biomolecular Chemistry, 2010 , vol. 8, # 18 p. 4059 - 4062

Safety Information

Download SDS
GHS Pictogram :
GHS No : GHS07
Signal Word : Warning
UN#: N/A
Class : N/A
Packing Group : N/A
Hazard Statements : H302-H315-H319-H335
Precautionary Statements : P261-P264-P270-P271-P280-P302+P352-P304+P340-P305+P351+P338-P330-P362+P364-P403+P233-P405-P501

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Computational Chemistry Data

  • TPSA   67.43
  • LogP   3.0133
  • H_Acceptors   4
  • H_Donors   2
  • Rotatable_Bonds   6

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