Activity:
Adrenomedullin (1-50), rat is a 50 amino acid peptide, which induces a selective arterial vasodilation via activation of CGRP1 receptor.
IC50 & Target: CGRP1 receptor[1]
In Vitro: Adrenomedullin (1-50), rat is a 50 amino acid peptide, which induces a selective arterial vasodilation via activation of CGRP1 receptor[1].
In Vivo: Adrenomedullin (1-50), rat causes a dose-dependent and endothelium-independent vasodilation on the arterial mesenteric vasculature, and this effect is inhibited by CGRP1 receptor antagonist. Adrenomedullin (1-50), rat activates CGRP1 receptor in the double-perfused mesenteric bed of the rat[1]. Adrenomedullin (1-50) ameliorates pulmonary vascular structural remodeling of hypoxic rats with incresed plasma NO and H(2)S concentrations. Adrenomedullin (1-50) also regulates the development of hypoxic pulmonary hypertension and hypoxic pulmonary vascular structural remodeling, through promoting NO and H(2)S production in hypoxic rats[2].
Protocol:
Animal Administration: Adrenomedullin (1-50), rat is formulated in PBS
[1].
[1]Rats
[1]
Male albino Wistar rats (250-350 g) are used in the assay. Following an equilibration period of 45 min, the
vasoactive effect of
Adrenomedullin (1-50), rat, rADM (11-50), acetylcholine (ACh), and bradykinin (BK) is evaluated. The perfusion pressure on both sides of the mesenteric circulation is increased by infusing either a sympathomimetic, methoxamine (100 μM), on the arterial side or a thromboxomimetic, U46619 (0.5 μM), on the venous side. When a plateau is reached, the agents (
Adrenomedullin (1-50), rat, etc.) are administered by bolus injections (
1 to 45 μL). Vasodilator responses are expressed as percent reduction of induced tone on both sides of the mesenteric vasculature. In some experiments, the effects of the CGRP1 receptor antagonist hCGRP
8-37 and of the nitric oxide synthase inhibitor L-NAME are evaluated. They are infused 15 and 30 min, respectively, before the administration of the agonists
[1].
References:
Qi JG, et al. Effect of adrenomedullin 1-50 on chronic hypoxic pulmonary hypertension in rats. Beijing Da Xue Xue Bao Yi Xue Ban. 2006 Apr 18;38(2):151-4.
Berthiaume N, et al. Rat adrenomedullin induces a selective arterial vasodilation via CGRP1 receptors in the double-perfused mesenteric bed of the rat. Can J Physiol Pharmacol. 1995 Jul;73(7):1080-3.
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