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CAS No. : 9014-00-0
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|Cat. No. :||CS-7681|
|CAS No. :||9014-00-0|
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Luciferase from Vibrio fischeri has also been used in a study to investigate the sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species.
Protocol:Cell Assay: Following CO2 asphyxiation, animals are perfused with 0.9% sodium chloride and then the liver and spleen are harvested and disrupted into single-cell suspensions. Liver lymphocytes are isolated on a 44% to 67% percoll gradient. Red blood cells in splenocyte suspensions are lysed using ACK lysis buffer. Cell suspensions are stimulated with 1 μg/mL Luciferase peptide plus 1 μg/mL GolgiPlug for 4 hr at 37°C. Animal Administration: Mice are anesthetized with 0.2 mL of ketamine cocktail i.p., and then given 0.1 mL of 28.5 mg/mL D-luciferin i.p. Animals are then imaged for a duration of 0.5 sec to 5 min using the imaging system. Luciferase activity levels are expressed in photons emitted per second per square centimeter, denoted as p/sec/cm2.
David M. Mofford, et al. Aminoluciferins Extend Firefly Luciferase Bioluminescence into the Near-Infrared and Can Be Preferred Substrates over d-Luciferin. J Am Chem Soc. 2014 Sep 24; 136(38): 13277-13282.
Kelly M, et al. Cellular Immune Response Against Firefly Luciferase After Sleeping Beauty–Mediated Gene Transfer In Vivo. Hum Gene Ther. 2014 Nov 1; 25(11): 955-965.
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