CAS No. : 866893-90-5
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|Cat. No. :||CS-0034791|
|CAS No. :||866893-90-5|
|M. Wt. :||368.48|
|Solubility:||DMSO: : ≥ 250 mg/mL|
Aftin-4 is an Amyloid-β42 (Aβ42) inducer. IC50 & Target: Amyloid-β In Vitro: Aftin-4 selectively and potently increases Aβ1-42 in N2a cells, primary neurons, and brain lysates, with an EC50 value around 30 μM. In Vivo: Aftin-4 increases Aβ1-42 levels in vivo in mice and provokes rapidly a sustained toxicity highly reminiscent of Alzheimer’s disease (AD). Aftin-4 is administered at increasing doses, between 3 and 20 nmol/mouse, into the lateral ventricle and animals are sacrificed at various time points, between 3 to 14 days after injection. The hippocampus is dissected out the contents in Aβ1-40 or Aβ1-42 is determined using a mouse ELISA assay. Aftin-4 dose-dependently and significantly increases Aβ1-42 content, up to +216% at the highest dose tested.
Protocol:Animal Administration: Aftin-4 is solubilized in DMSO 40% in distilled water (i.p.).Mice
Male Swiss OF-1 mice, aged 7-9 weeks and weighing 32±2 g are used. Aftin-4 is solubilized in DMSO at a concentration of 3 mg/mL and stored at -20°C until use. Aftin-4 is administered intracerebroventricularly (i.c.v. ), with a Hamilton microsyringe equipped with a 3-mm needle in a final volume of 3 μL per mouse. The injection coordinates are -0.4 mm with respect to bregma, 1.00mm to the right from the central, and 2.50mm in depth. Aftin-4 is also injected intraperitoneally (i.p.). Aftin-4 is solubilized in DMSO 40% in distilled water at 2, 6 or 20 mg/mL and administered in a final volume of 100 μL/20 g body weight.
Meunier J, et al. Brain toxicity and inflammation induced in vivo in mice by the amyloid-β forty-two inducer aftin-4, a roscovitine derivative. J Alzheimers Dis. 2015;44(2):507-24.
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