Thymus factor X
CAS No. : 78310-77-7

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  • Data Sheet

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Name: Thymus factor X; TFX-Jelfa
Cat. No. : CS-0012208
CAS No. : 78310-77-7
Formula:
M. Wt. :
Solubility: DMSO

Activity:

Thymus factor X is a biologically active polypeptide which can be used as a natural stimulator of lymphocyte function. In Vivo: The percentage of necrotic lymphocytes in the spleen in Group I (infected with T. spiralis and treated with Thymus factor X (TFX)) is significantly lower than in the control group on day 7, and approximately the same as in the control group for the rest of the experiment. The percentage of necrotic lymphocytes in muscle tissue in Group I is approximately the same as in the control group on day 7, significantly lower on days 14 and 21 (p<0.0002), approximately the same on days 28 and 35, significantly lower on day 42, and significantly higher on day 60 (p<0.005)[1].

Protocol:

Animal Administration: Thymus factor X is dissolved in saline immediately before use.[1]The experiment is carried out using 134 mice divided into three groups: Group I: 46 mice infected with T. spiralis and treated with Thymus factor X (TFX); Group II: 42 uninfected mice treated with Thymus factor X; and Control group: 46 mice infected with T. spiralis and not treated with Thymus factor X. Thymus factor X is administered subcutaneously at a dose of 15 mg/kg. The first dose is administered on the third day before the mice are infected with T. spiralis. The dose is repeated at 24 h intervals until the thirteenth day after infection. In all, 17 doses are administered. On days 7, 14, 21, 28, 35, 42, and 60 after infection, six mice from each group are sacrificed using halothane. The spleen, mesenteric lymph nodes and masseter muscles are excised from each mouse and transferred to sterile phospho-buffered saline at 4°C[1].

References:

Piekarska J, et al. Trichinella spiralis: Effect of thymus factor X on apoptosis and necrosis in mice. Exp Parasitol. 2009 Oct;123(2):128-33.

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