CAS No. : 61909-81-7
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|Cat. No. :||CS-0040442|
|CAS No. :||61909-81-7|
|M. Wt. :||374.60|
Solutol HS-15 is a permeability enhancer. In Vitro: Solutol HS-15 is a permeability enhancer. A general decline in cell metabolic activity is seen for all three tested cell lines as the concentration of Solutol HS-15 solution is increased. Results demonstrate that Solutol HS-15 promotes the apical-to-basolateral translocation of a model protein (insulin) across Calu-3 monolayers when applied at 37°C. Furthermore there is no statistically significant increase in insulin permeability in the presence of Solutol HS-15, relative to the respective control, at 4°C. Solutol HS-15 treated cells show a statistically significant increase in permeability towards lower molecular weight permeants (5 to 22 kDa) and an absence of a statistically significant increase in permeability of proteins with larger molecular weight, namely albumin and IgG (P>0.05 relative to control).
Protocol:Cell Assay: For both MTS and LDH cell viability assays, cells are seeded at a density of 1×104 cells on 96-well plates and cultured for a minimum of 24 h. Solutol HS-15 (in 25 mM HBSS:HEPES, pH 7.4) solutions are applied at concentrations above and below the critical micelle concentration (CMC) (0.01 mM to 20 mM) and cytotoxicity is determined using the MTS and LDH assays. 25 mM HBSS:HEPES is used as a negative control and Triton-X 100 (3 mM) as a positive control. Following 3 h incubation, the MTS reagent in media and LDH working solution are added to the cells and the cells are incubated for a further 3 h at 37°C.
Shubber S, et al. Mechanism of mucosal permeability enhancement of CriticalSorb? (Solutol? HS15) investigated in vitro in cell cultures. Pharm Res. 2015 Feb;32(2):516-27.
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