LM22B-10
CAS No. : 342777-54-2

Size Stock Price Quantity Add to Cart Quotation Online
2mg In-stock USD 120.0
5mg In-stock USD 180.0
10mg In-stock USD 300.0
50mg In-stock USD 1092.0
100mg In-stock USD 1920.0
Products are for research use only. Not for human use. We do not sell to patients.

Bulk Inquiry

Collapse
Your information is safe with us. * Required Fields
  Salutation:
* Country or Region:
* Applicant name:
* Organization Name:
* Email address:
* Requested quantity:
* Phone number:
  Remarks:
 
Name, 
Organization name, 
Email address, 
Requested quantity, 
Phone number, 
  • Data Sheet

  • Introduction

  • SDS

  • COA & Spectra

Name: LM22B-10; 
Cat. No. : CS-7663
CAS No. : 342777-54-2
Formula: C27H33ClN2O4
M. Wt. : 485.01
Solubility: DMSO: ≥ 150 mg/mL

Activity:

LM22B-10 is an activator of TrkB/TrkC neurotrophin receptor, and can induce TrkB, TrkC, AKT and ERK activation in vitro and in vivo. In Vitro: LM22B-10 exhibits maximum neurotrophic survival activity levels that are higher than those maximally achieved with BDNF (53 ± 7.2% above BDNF at 0.7 nM) and NT-3 (91 ± 8.6% above NT-3 at 0.7 nM) with an EC50 value of 200-300 nM. LM22B-10 (1000 nM) induces neurites of significantly larger average lengths, up to -40 μM. LM22B-10 (250-2000 nM) binds to TrkB-Fc and TrkC-Fc in a dose-dependent manner. LM22B-10 inhibits binding of BDNF to TrkB-expressing cells and NT-3 to TrkC-expressing cells. LM22B-10 promotes cell survival and functions preferentially through TrkB and TrkC. LM22B-10, but not BDNF or NT-3, promotes neurite outgrowth in an inhibitory environment. LM22B-10 induces patterns of Trk and downstream signaling activation that are distinct from those of BDNF and NT-3. LM22B-10 also induces TrkB, TrkC, AKT and ERK activation in hippocampal neurons in culture[1]. In Vivo: LM22B-10 (0.5 mg/kg) activates TrkB, TrkC, AKT and ERK in C57BL/6J mice. LM22B-10 (50 mg/kg, i,p,) shows increased phosphorylation at TrkBY817 and TrkCY820. LM22B-10 activates synaptic TrkB and TrkC and increases pre- and post-synaptic proteins and spine density in aged mice[1].

Protocol:

Cell Assay: LM22B-10 is dissolved in 0.04 N HCl prior to dilution (1:10,000) in culture medium.[1]Mouse NIH-3T3 cells, mouse NIH-3T3 cells expressing TrkA (NIH-3T3-TrkA) or p75NTR (NIH-3T3-p75NTR), and NIH-3T3 cells expressing TrkB (NIH-3T3-TrkB) or TrkC (NIH-3T3-TrkC) are propagated in DMEM supplemented with 10% FBS and 200-400 μg/mL Geneticin (for Trk-expressing cells) or 400 μg/mL hygromycin (for p75NTR-expressing cells). Cells are seeded into 24-well plates (30,000 cells/well) and cultured in medium consisting of 50% PBS and 50% DMEM without supplements. Following exposure to growth factors (0.7 nM) or 1000 nM LM22B-10 for 72-96 h, cells are suspended in 50 μL lysis buffer, transferred to opaque 96-well culture plates and survival is measured using the ViaLight Assay.

References:

Yang T, et al. A small molecule TrkB/TrkC neurotrophin receptor co-activator with distinctive effects on neuronal survival and process outgrowth. Neuropharmacology. 2016 Nov;110(Pt A):343-61.

Enter Lot Number:

We apologize for the inconvenience. The Certificate of Analysis you requested is not currently available on-line:

1. The Lot Number you requested may have been entered incorrectly. How to get a Lot Number

2. The COA of the Lot you requested has not been added to our database.

Browsing History
    Tags:LM22B-10 supplier,LM22B-10 purchase,LM22B-10 manufacturer,LM22B-10 sigma,LM22B-10 distributor,cost,LM22B-10 buy,LM22B-10 for sale
    Please enter your email address
    Organization name: Email:

    Submit success

    Message
    No information on this batch number
    How to get a Lot Number

    Lot numbers can be found on the outside label of a product:

    ChemScene Label