FT827
CAS No. : 1959537-86-0

FT827
Cat. No. : CS-0039475
M. Wt. : 548.61
Size Stock Price Quantity Add to Cart Quotation Online
5mg In-stock USD 650.0
10mg In-stock USD 1100.0
25mg In-stock USD 2200.0
50mg In-stock USD 3500.0
100mg In-stock USD 4900.0
Products are for research use only. Not for human use. We do not sell to patients.

Bulk Inquiry

Collapse
Your information is safe with us. * Required Fields
  Salutation:
* Country or Region:
* Applicant name:
* Organization Name:
* Email address:
* Requested quantity:
* Phone number:
  Remarks:
 
Name, 
Organization name, 
Email address, 
Requested quantity, 
Phone number, 
  • Data Sheet

  • Introduction

  • SDS

  • COA & Spectra

Name: FT827; 
Cat. No. : CS-0039475
CAS No. : 1959537-86-0
Formula: C27H28N6O5S
M. Wt. : 548.61
Solubility: DMSO

Activity:

FT827 is a selective and covalent ubiquitin-specific protease 7 (USP7) inhibitor with an IC50 of 52 nM. IC50 & Target: IC50: 52 nM (USP7)[1] In Vitro: FT827 features a vinylsulfonamide moiety that covalently modifies the catalytic Cys223 of USP7 and inhibits the enzyme with Ki and Kd of 7.8 and 4.2 μM, respectively. FT827 exclusively inhibit USP7 in a panel of 38 deubiquitinases (DUBs) from diverse families. FT827 inhibits USP7 probe reactivity with IC50s of 0.1-2 µM, confirming 10 to 100-fold higher potency as compared to P22077 in crude cell extracts or with intact MCF7 breast cancer cells, followed by incubation with the ubiquitin active site suicide probe haemagglutinin (HA)-tagged ubiquitin bromoethyl (HA-UbC2Br)[1].

Protocol:

Kinase Assay: [1]To determine compound IC50s, FT827 is diluted in 100% DMSO in three-fold 12- point dilution series from 100 µM. 100 nL of 100-fold concentrated solutions are dispensed into black 384-well plate. 25 nM ubiquitin-rhodamine 110, along with recombinant USP7CD (3 nM), or USP7C-term (30-125 pM, depending on batch activity) are added and the plates incubated at room temperature for 1 h. The reaction is terminated by adding 2.5 µL citric acid to a final concentration of 10 mM prior to measuring fluorescence intensity on a Pherastar with a 485 nm excitation/520 nm emission optic module[1].

References:

Turnbull AP, et al. Molecular basis of USP7 inhibition by selective small-molecule inhibitors. Nature. 2017 Oct 26;550(7677):481-486.

Enter Lot Number:

We apologize for the inconvenience. The Certificate of Analysis you requested is not currently available on-line:

1. The Lot Number you requested may have been entered incorrectly. How to get a Lot Number

2. The COA of the Lot you requested has not been added to our database.

Browsing History
    Tags:FT827 supplier,FT827 purchase,FT827 manufacturer,FT827 sigma,FT827 distributor,cost,FT827 buy,FT827 for sale
    Please enter your email address
    Organization name: Email:

    Submit success

    Message
    No information on this batch number
    How to get a Lot Number

    Lot numbers can be found on the outside label of a product:

    ChemScene Label