CAS No. : 150347-59-4
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|Name:||CFSE; 5(6)-Carboxyfluorescein diacetate succinimidyl ester; CFDA-SE; 5(6)-CFDA N-succinmidyl ester|
|Cat. No. :||CS-7677|
|CAS No. :||150347-59-4|
|M. Wt. :||557.46|
CFSE is a fluorescent dye which can track the cell division. In Vitro: CFSE is a fluorescent dye which can track the cell division. To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, labeling them with CFSE, and adding them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFSE-labeling immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFSE-labeling immune cells.
Protocol:Cell Assay: Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×106 CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert.
Cinthia C. Stempin, et al. GRAIL and Otubain-1 are Related to T Cell Hyporesponsiveness during Trypanosoma cruzi Infection. PLoS Negl Trop Dis. 2017 Jan; 11(1): e0005307.
Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017; 6(7): e1333210.
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