Adrenomedullin (AM) (1-52), human
CAS No. : 148498-78-6

Adrenomedullin (AM) (1-52), human
Cat. No. : CS-0043650
M. Wt. : 6028.82
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Name: Adrenomedullin (AM) (1-52), human; Human adrenomedullin-(1-52)-NH2
Cat. No. : CS-0043650
CAS No. : 148498-78-6
Formula: C264H406N80O77S3
M. Wt. : 6028.82
Solubility: H2O

Activity:

Adrenomedullin (AM) (1-52), human is a 52-amino acid peptide, which affects cell proliferation and angiogenesis in cancer. In Vitro: To explore the effect of Adrenomedullin (AM) (1-52), human on astroglioma cells, CRT-MG cells are incubated in the absence or presence of Adrenomedullin (AM) (1-52), human (ADM1-52) for 48 h in a medium containing 1% FBS, and wound-healing assay is performed. The number of cells migrating to the wound region significantly increases in the ADM1-52-treated cells, in a dose-dependent manner, compared to the untreated cells[1].

Protocol:

Cell Assay: [1]CRT-MG cells are scraped off the bottom of a culture plate using a pipette tip to create a cell-free area. The cell culture is washed with PBS to remove cell debris and then incubated with oncostatin M (OSM), Adrenomedullin (AM) (1-52), human (ADM1-52; 0.1 μM and 0.5 μM) for 48 h in 1% FBS DMEM. The wound area is photographed after scratching for control. The number of cells migrating into the initial wound area is counted at 48 h after scratching[1].

References:

Lim SY, et al. Transcriptional regulation of adrenomedullin by oncostatin M in human astroglioma cells: implications for tumor invasion and migration. Sci Rep. 2014 Sep 23;4:6444.

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